ABSTRACT
OBJECTIVE:To investigate the effects of NOS1AP rs12742393 A/C gene polymorphism on lipid-regulating re-sponse of rosuvastatin calcium. METHODS:Two hundred and tuirty six patients with coronary heart disease(CHD)were selected from cardiology department of our hospital during Jan. 2014-Jun. 2015,and then given rosuvastatin calcium and other symptomatic treatment for 12 weeks. Polymorphism of NOS1AP rs12742393 A/C was detected by PCR-RFLP. The levels of TG,TC,HDL-C and LDL-C were detected by photoelectric colorimetry before treatment and 4,12 weeks after treatment. The serum relationship of genotype with the level of blood lipid was analyzed. RESULTS:Among 236 CHD patients,there were 131 cases of AA genotype (55.5%),98 cases of AC genotype(41.5%) and 7 cases of CC genotype(3.0%);genotype and allele frequencies met the Har-dy-Weinberg balance(P>0.05). There were 132 patients with normal blood lipid and 104 patients with hypercholesterolemia;there was statistical significance in genotype and allele frequencies (P0.05). 4th and 12th week after treatment,the levels of TG,TC and LDL-C in different genotypes were all de-creased significantly;4th week after treatment,the level of LDL-C in AC+CC genotype was significantly lower than AA genotype, and the change compared to before treatment was significantly more than AA genotype,with statistical significance (P0.05). CONCLUSIONS:NOS1AP rs12742393 A/C gene polymorphism is associated with CHD complicated with hypercholesterolemia;the C allele of NOS1AP rs12742393 may strengthen the response of CHD patients with hy-percholesterolemia to rosuvastatin calcium through influencing the level of LDL-C.
ABSTRACT
To investigate the role of polo-like kinase -1 [PLKl] in the first cleavage of a zygote in culture. This experiment took place in the Reproductive Medicine Center of Tongji Hospital, Wuhan, China, from 1st June 2009 to 20th November 2009. First, we detected the expression of PLKl during the first zygotic division by using Western blotting, and then we reduced the expression of PLKl during the first zygotic division by ribonucleic acid [RNA] interference [including 4 groups: PLKl small interfering RNA [siRNA], siRNA control, mock transfection, and only zona pellucida [ZP] removal], finally we evaluated and compared the first cleavage rates of the 4 groups. The expression of PLKl peaked in the first M phase of zygotic cleavage [3 samples/group, 100 zygotes/sample]. The relative amount of PLKl of the mouse zygotes was reduced significantly after siRNA transfection. The first cleavage rate of the PLKl siRNA group was significantly less than that of other groups [siRNA control, mock transfection, and only ZP removal, p=0.000]. The PLKl plays a crucial role during the first cleavage of one-cell embryos, and the zygotes are unable to divide successfully without functional PLKl